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| Robalino, Javier; Chapman, Robert W.; De La Vega, Enrique; O'Leary, Nuala A.; Gorbach, Danielle M.; Du, Zhi-qiang; Rothschild, Max F.; Browdy, Craig L.; Warr, Gregory; Labreuche, Yannick. |
| This chapter contains sections titled: Introduction EST Collection as an Approach to Gene Discovery in Shrimp Medium- to High-Throughput Studies of Differential Expression and Gene Discovery RNAi-Based Applications in Shrimp Aquaculture: From Reverse Genetics to Control of Diseases Markers, Genetic Maps, and Large Insert Genomic Libraries in Shrimp Analytical Challenges in Genomics and Genetics of Shrimp Concluding Remarks Acknowledgments References |
| Tipo: Text |
Palavras-chave: Penaeid shrimp EST collection; Gene discovery gene expression “snapshot” gametogenesis abiotic stress responses differential expression cloning RNAi-based applications; Shrimp targeted gene silencing RNAi; And gene function genetic markers in breeding. |
| Ano: 2012 |
URL: http://archimer.ifremer.fr/doc/00070/18149/15775.pdf |
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| Labreuche, Yannick; O'Leary, Nuala A.; De La Vega, Enrique; Veloso, Artur; Gross, Paul S.; Chapman, Robert W.; Browdy, Craig L.; Warr, Gregory W.. |
| injection of non-specific dsRNA initiates a broad-spectrum innate antiviral immune response in the Pacific white shrimp, Litopenaeus vannamei, however, the receptor involved in recognition of this by-product of viral infections remains unknown. In vertebrates, dsRNA sensing is mediated by a class of Toll-like receptors (TLRs) and results in activation of the interferon system. Because a TLR (lToll) was recently characterized in L. vannamei, we investigated its potential role in dsRNA recognition. We showed that injection of non-specific RNA duplexes did not modify lToll gene expression. A reverse genetic approach was therefore implemented to study its role in vivo. Silencing of lToll did not impair the ability of nonspecific dsRNA to trigger protection... |
| Tipo: Text |
Palavras-chave: Shrimp; Virus; RNA interference; Invertebrate immunity; Innate immune response; Toll like receptor; DSRNA. |
| Ano: 2009 |
URL: http://archimer.ifremer.fr/doc/2009/publication-6428.pdf |
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